TL;DR — TB-500 is a synthetic fragment of Thymosin Beta-4 (TβMin4), a naturally occurring 43-amino-acid protein involved in actin sequestration and cell motility. Research has investigated its effects on actin polymerisation, wound healing signalling, fibrosis reduction, and inflammatory pathway modulation. TB-500 is supplied strictly as a research compound. The B.A.B.E LABS catalogue includes TB-500 in:
What is TB-500?
TB-500 is the common research name for a synthetic N-acetylated fragment of Thymosin Beta-4 (TβMin4), typically the biologically active region spanning residues 17–23 (LKKTETQ). The parent protein, Thymosin Beta-4, is a 43-amino-acid peptide first isolated from the thymus gland and subsequently found in virtually all mammalian cells and tissues.
TβMin4's primary cellular function is actin sequestration: it binds monomeric (G-actin) molecules and regulates their availability for polymerisation into the actin cytoskeleton. This is the molecular lever behind most of the phenotypes TB-500 is studied for.
Mechanism of action:
Actin regulation
Research has characterised TβMin4 as the principal G-actin sequestering protein in mammalian cells. By regulating the free G-actin pool, it influences cell migration, shape change, and proliferation — all processes central to tissue repair.
Cell migration and wound healing models
Published work has investigated TB-500 / TβMin4 in corneal wound models, dermal wound models, and cardiac ischaemia models. Studies have reported accelerated epithelial migration, endothelial cell recruitment, and reduced scar tissue formation in preclinical settings.
Anti-fibrotic signalling
Several papers have examined TβMin4's effect on TGF-β pathway signalling and collagen organisation, with some studies reporting reduced fibrotic deposition in post-injury tissue.
Inflammatory pathway modulation
Research has investigated TβMin4's interaction with the NF-κB inflammatory cascade, reporting attenuated pro-inflammatory cytokine expression in certain experimental models.
Hair follicle and dermal research
TβMin4 has been studied in hair follicle stem cell models, where it has been reported to influence follicular cycle progression.
Why TB-500 is paired with BPC-157:
BPC-157 and TB-500 act through distinct, non-overlapping mechanisms:
The WOLVERINE blend combines the two because preclinical research suggests their mechanisms may be complementary across the tissue-repair cascade — from initial inflammation through to structural remodelling.
Purity, testing, COA:
- HPLC ≥99% confirmed per batch
- Mass spectrometry verifies the expected molecular weight of the fragment supplied
- Certificate of Analysis accompanies every batch — see the COA Page.
Storage and reconstitution:
- Lyophilised: –20°C, protected from light
- Reconstituted: 2–8°C, use within the COA window
Where TB-500 fits in the B.A.B.E LABS catalogue:
Research-use disclaimer:
TB-500 is supplied strictly for in-vitro and laboratory research. Not for human consumption. Not TGA-approved for therapeutic use.
FAQs:
Is TB-500 the same as Thymosin Beta-4?
TB-500 is a synthetic fragment of TβMin4 — usually the 17-23 active region. The published literature often uses the names interchangeably in pharmacology contexts, but strictly, TβMin4 refers to the full 43-amino-acid protein.
Why is the dose per vial lower than some BPC-157 vials?
TB-500 has a higher molecular weight than BPC-157, so milligram-per-milligram comparisons aren't directly meaningful. Always work from the COA and published research protocols for the specific fragment you're using.
Why store at –20°C when unreconstituted?
Lyophilised peptides remain stable for 24+ months at –20°C. Higher temperatures accelerate deamidation and oxidation of labile residues. Once reconstituted, 2–8°C is sufficient for short-term storage.